Archive | April 2012
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After quite some time on vacation (10 days!) it is kinda hard to return to the bench. It’s not that I am lazy, far from it, its just that the mindset is tuned on different waves and all of a sudden, you need to shift yourself from 0 to 100 Mph. Well, I’ve decided this […]
In many cases, even if we have a relatively purified protein, we would want to add an additional polishing step in the form of a size exclusion chromatography (SEC) or gel filtration. In this post I will discuss some considerations and tips in regard to maximizing the use of SEC.