In previous posts I have discussed methods to grow protein crystals and how to monitor their growth. Yet, in many cases the first screen(s) trials will not yield any protein crystals. In such a case, what strategies one should explore on the path for protein crystals? In this and later posts I will discuss these rescue strategies and how they can help in improving your chance to obtain the sought protein crystal.
List of common rescues methods
- Subcloning (following a rational biochemical approach)
- Lysine methylation
- Surface entropy reduction
- In-situ proteolysis
- Homologue screen
In the following posts I will discuss each method separately, getting into details were possible.