A century ago, most people worked at their home proximity, most of their day was centered on labor and less on traveling. Today, with the expanding globalization culture and the availability of fast transportation means, many people commute tens and even over hundred kilometers from their home to their place of work. However, fast as […]
After quite some time on vacation (10 days!) it is kinda hard to return to the bench. It’s not that I am lazy, far from it, its just that the mindset is tuned on different waves and all of a sudden, you need to shift yourself from 0 to 100 Mph. Well, I’ve decided this […]
In the previous post I have focused mainly on preparing and performing the first steps in the purification of a recombinant protein. In this section I will discuss a bit further about Nickel affinity chromatography via prepack column, about gradient elution and give some advice on improving purification of dirty preps.
Everyone knows it is crucial to have breaks from time to time (if you can, do a 10 minutes break every working hour). So I have decided to share with you this funny vid about common phrases graduate student tell their PIs, colleagues and even their spouse…hope you enjoy this share!
So now that I have finished writing the BSF grant, I am finally free to get back to the bench and start kicking some serious work. One of my “on the shelf proteins” is a small protein less than 20 kDa which expresses poorly – so, in this post I will discuss how to boost […]
A couple of days ago I have seen a comment on twitter made by @Bam294 – here it goes: Now, putting aside the fact that it is a wrong decision to transfer samples from -80 degrees to plus 4 degrees, it reminded me of the importance of order and documentation of freezers sample, especially those […]